Anti-EDG1 Polyclonal Antibody is a Rabbit antibody targeting EDG1. Anti-EDG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Human,Mouse,Rat (predicted:Dog,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (TMAB-00588) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (TMAB-00588) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
HUVEc (Human) Cell Lysate at 30 μg
Primary: Anti-EDG1 (TMAB-00588) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 54 kDa
4. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-EDG1 (TMAB-00588) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human EDG1/CD363

Human

Associated Proteins,Endothelial Cell Markers,Associated Proteins,Endothelial,GPCR,Lipid Signaling