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顶部生物活性储存和溶解信息计算器

Anti-E2F1 Antibody (2K279)

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Anti-E2F1 Antibody (2K279)
产品编号 TMAC-01210

Anti-E2F1 Antibody (2K279) 是一种 Rabbit 抗体，靶向 E2F1。Anti-E2F1 Antibody (2K279) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-E2F1 Antibody (2K279)

Anti-E2F1 Antibody (2K279)

Rating icon 还可以
Anti-E2F1 Antibody (2K279)
产品编号 TMAC-01210

Anti-E2F1 Antibody (2K279) 是一种 Rabbit 抗体，靶向 E2F1。Anti-E2F1 Antibody (2K279) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-E2F1 Antibody (2K279) is a Rabbit antibody targeting E2F1. Anti-E2F1 Antibody (2K279) can be used in WB,ICC/IF,IHC,IP,FCM.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
3. ICC staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
7. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
8. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
应用
WB
ICC/IF
IHC
IP
FCM
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q01094
化学信息
分子量Theoretical: 55 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

Transcription factor E2F1Retinoblastoma-binding protein 3 (RBBP-3)Retinoblastoma-associated protein 1 (RBAP-1)RBBP-3RBBP3RBBP 3RBAP-1RBAP1pRB-binding protein E2F-1PBR3PBR 3E2F-1E2F1E2F 1

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