Anti-DYKDDDDK (FLAG epitope Tag) Antibody (5I361) is a Mouse antibody targeting DYKDDDDK (FLAG epitope Tag). Anti-DYKDDDDK (FLAG epitope Tag) Antibody (5I361) can be used in ICC/IF.

Monoclonal Mouse IgG2b

5I361

other

Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38(Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).

ICC/IF

ICC-IF: 1:50-1:1000

Monoclonal

Unconjugated

HRP

2

A synthetic peptide corresponding to the flag-tag sequence (DYKDDDDK).

other