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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-DUSP10 Polyclonal Antibody

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货号 TMAB-05338

Anti-DUSP10 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DUSP10。Anti-DUSP10 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

Anti-DUSP10 Polyclonal Antibody

Anti-DUSP10 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-05338

Anti-DUSP10 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DUSP10。Anti-DUSP10 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-DUSP10 Polyclonal Antibody is a Rabbit antibody targeting DUSP10. Anti-DUSP10 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DUSP10) Polyclonal Antibody, Unconjugated (TMAB-05338) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DUSP10) Polyclonal Antibody, Unconjugated (TMAB-05338) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DUSP10) Polyclonal Antibody, Unconjugated (TMAB-05338) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DUSP10) Polyclonal Antibody, Unconjugated (TMAB-05338) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DUSP10) Polyclonal Antibody, Unconjugated (TMAB-05338) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DUSP10) Polyclonal Antibody, Unconjugated (TMAB-05338) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
应用IHC-PIHC-FrIF
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus.
组织特异性Detected in brain.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. MKP-5 preferentially binds to p38, but also to SAPK/JNK. It is ubiquitously expressed and localizes to both the cytoplasm and the nucleus. MKP-5 has been implicated in cell proliferation and apoptosis, tumor invasion and immune responses.
抗原信息
免疫原
KLH conjugated synthetic peptide: human DUSP10/MKP5
抗原种属
Human
基因名称
DUSP10
基因ID
11221
蛋白名称
Dual specificity protein phosphatase 10
Uniprot ID
Q9Y6W6
功能
Involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.
化学信息
分子量Theoretical: 53 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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