Anti-DSPP Polyclonal Antibody 2 is a Rabbit antibody targeting DSPP. Anti-DSPP Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated (TMAB-00574) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: SW480 (Human) Cell Lysate at 30 μg
Lane 3: Du145 (Human) Cell Lysate at 30 μg
Primary: Anti-DSPP (TMAB-00574) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 130 kDa
3. Sample:
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-DSPP (TMAB-00574) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/129 kDa
Observed band size: 129 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Dentin sialoprotein

Human

Cell differentiation,Bone,Other ECM Proteins,Bone,Calcium Binding Proteins