Anti-Doublecortin/DCX Polyclonal Antibody is a Rabbit antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Polyclonal Antibody can be used in WB,ICC/IF.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-DCX/Doublecortin (TMAB-00564) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
2. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (DCX/Doublecortin) polyclonal Antibody, Unconjugated (TMAB-00564) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; ICC/IF: 1:100

Polyclonal

KLH conjugated synthetic peptide: human DCX

Human

DCX

Neuronal migration protein doublecortin

Microtubules,Growth Cone,Soma marker,Neuronal

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration.