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Anti-Doublecortin/DCX Polyclonal Antibody
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产品编号 TMAB-00564
Anti-Doublecortin/DCX Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Doublecortin/DCX。Anti-Doublecortin/DCX Polyclonal Antibody 可用于 WB,ICC/IF。
Anti-Doublecortin/DCX Polyclonal Antibody
Anti-Doublecortin/DCX Polyclonal Antibody
还可以产品编号 TMAB-00564
Anti-Doublecortin/DCX Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Doublecortin/DCX。Anti-Doublecortin/DCX Polyclonal Antibody 可用于 WB,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Doublecortin/DCX Polyclonal Antibody is a Rabbit antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Polyclonal Antibody can be used in WB,ICC/IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-DCX/Doublecortin (TMAB-00564) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa 2. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (DCX/Doublecortin) polyclonal Antibody, Unconjugated (TMAB-00564) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; ICC/IF: 1:100 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Cell projection. Note=Localizes at neurite tips. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Neuronal Marker Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express, a marker for mature neurons. Due to the nearly exclusive expression of DCX in developing neurons, this protein has been used increasingly as a marker for neurogenesis. Indeed, the levels of DCX expression increase in response to exercise, which occurs in parallel with increased BrdU labelling, currently a "gold standard" in measuring neurogenesis. |
| 免疫原 | KLH conjugated synthetic peptide: human DCX |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Microtubules,Growth Cone,Soma marker,Neuronal |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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