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Anti-Dopamine D3 receptor Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Dopamine D3 receptor。Anti-Dopamine D3 receptor Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Dopamine D3 receptor Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Dopamine D3 receptor。Anti-Dopamine D3 receptor Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
| 产品描述 | Anti-Dopamine D3 receptor Polyclonal Antibody is a Rabbit antibody targeting Dopamine D3 receptor. Anti-Dopamine D3 receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 反应种属 | Human,Mouse,Rat |
| 验证活性 | 1. Sample: HepG2 Cell Lysate at 30 μg U251 Cell Lysate at 30 μg Primary: Anti-Dopamine D3 receptor (TMAB-05286) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kD 2. Sample: Brain (Mouse) Lysate at 40 μg Brain (Rat) Lysate at 40 μg Primary: Anti-Dopamine D3 receptor (TMAB-05286) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kD 3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (TMAB-05286) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (TMAB-05286) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining. |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. |
| 组织特异性 | Brain. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is expressed in phylogenetically older regions of the brain, suggesting that it plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). Also known as: dopamine D3 receptor; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1. |
| 免疫原 | KLH conjugated synthetic peptide: mouse DRD3 |
| 抗原种属 | Mouse |
| 基因名称 | DRD3 |
| 基因ID | |
| 蛋白名称 | D(3) dopamine receptor |
| Uniprot ID |
| 分子量 | Theoretical: 44 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |