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Anti-DNMT1 Antibody (5Y204)
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产品编号 TMAC-01168
别名 MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM
Anti-DNMT1 Antibody (5Y204) 是一种 Rabbit 抗体，靶向 DNMT1。Anti-DNMT1 Antibody (5Y204) 可用于 WB,ICC/IF,IHC。
Anti-DNMT1 Antibody (5Y204)
Anti-DNMT1 Antibody (5Y204)
还可以产品编号 TMAC-01168 别名 MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM
Anti-DNMT1 Antibody (5Y204) 是一种 Rabbit 抗体，靶向 DNMT1。Anti-DNMT1 Antibody (5Y204) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-DNMT1 Antibody (5Y204) is a Rabbit antibody targeting DNMT1. Anti-DNMT1 Antibody (5Y204) can be used in WB,ICC/IF,IHC. |
| 别名 | MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Dnmt1 on HepG2 cells lysates using anti-Dnmt1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Dnmt1 antibody. Counter stained with hematoxylin. 3. ICC staining Dnmt1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Dnmt1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Dnmt1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 183 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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