Anti-DNA Ligase 1 Antibody (4I673) is a Rabbit antibody targeting DNA Ligase 1. Anti-DNA Ligase 1 Antibody (4I673) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P.

IgG

4I673

Human,Rat,Mouse

1. 4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (DNA Ligase 1) monoclonal Antibody, unconjugated (TMAB-05209) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-DNA Ligase 1 antibody (TMAB-05209, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase 1 Monoclonal Antibody, Unconjugated (TMAB-05209) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200

Monoclonal

A synthesized peptide: human LIG1

Human

LIG1

DNA ligase 1

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.