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Anti-DLL4 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 DLL4。Anti-DLL4 Polyclonal Antibody 可用于 WB。
Anti-DLL4 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 DLL4。Anti-DLL4 Polyclonal Antibody 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-DLL4 Polyclonal Antibody is a Rabbit antibody targeting DLL4. Anti-DLL4 Polyclonal Antibody can be used in WB. |
Ig Type | IgG |
交叉反应 | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse) |
验证活性 | 1. Sample: Lane 1: Human K562 cell Lysates Lane 2: Human THP-1 cell Lysates Lane 3: Human 293T cell Lysates Primary: Anti-DLL4 (TMAB-00554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa 2. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Delta 4 Polyclonal Antibody, Unconjugated (TMAB-00554) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
应用 | |
推荐剂量 | WB: 1:500-2000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Membrane; Single-pass type I membrane protein (Probable). |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged-2 and Delta. While blocking the differentiation of progenitor cells into the B-cell lineage, Delta promotes the emergence of a population of cells with T cell/NK-cell characteristics. The protein is a membrane protein expressed in heart, pancreas, brain and muscle during gastrulation and early organogenesis and in adult heart and lung. Delta-4 is a membrane protein that activates Notch-1 and Notch-4. It is expressed in a wide range of adult and fetal tissues, especially in vascular endothelium. |
免疫原 | KLH conjugated synthetic peptide: human DLL4 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Cell differentiation,Cell Differentiation,Notch Pathway |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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