Anti-DFNA5 Polyclonal Antibody is a Rabbit antibody targeting DFNA5. Anti-DFNA5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Cow,Sheep)

1. Sample:
Small intestine (Mouse) Lysate at 40 μg
Primary: Anti-DFNA5 (TMAB-00541) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
2. Protein: intestinal (mouse) lysate at 40 μg;
Primary: rabbit Anti-DFNA5 (TMAB-00541) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000;
Predicted band size: 54 kDa
Observed band size: 54 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DFNA5) Polyclonal Antibody, Unconjugated (TMAB-00541) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DFNA5

Human

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