Anti-Destrin Antibody (6G799) is a Rabbit antibody targeting Destrin. Anti-Destrin Antibody (6G799) can be used in FCM, ICC/IF.

IgG

6G799

Human (predicted:Mouse,Rat)

1. Western blot analysis of (1) HeLa cell lysate; (2) NIH/3T3 cell lysate. Using Destrin (TMAB-05105) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Destrin) monoclonal Antibody, unconjugated (TMAB-05105) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-Destrin antibody (TMAB-05105, 1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200

Monoclonal

A synthesized peptide: human Destrin

Human

DSTN

Destrin

Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.