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别名 Prolia Xgeva, Prolia, AMG-162, AMG 162
Anti-Denosumab Antibody (5H762) 是一种 Mouse 抗体,靶向 Denosumab。Anti-Denosumab Antibody (5H762) 可用于 ELISA, Competitive ELISA。
Anti-Denosumab Antibody (5H762) 是一种 Mouse 抗体,靶向 Denosumab。Anti-Denosumab Antibody (5H762) 可用于 ELISA, Competitive ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 40 μg | ¥ 1,609 | 5日内发货 |
| 产品描述 | Anti-Denosumab Antibody (5H762) is a Mouse antibody targeting Denosumab. Anti-Denosumab Antibody (5H762) can be used in ELISA, Competitive ELISA. |
| 别名 | Prolia Xgeva, Prolia, AMG-162, AMG 162 |
| Ig Type | IgG1,κ |
| 克隆号 | 5H762 |
| 特异性 | Denosumab |
| 验证活性 | 1. ELISA binding of Anti-Denosumab Antibody (5H762) (TMAS-00032) with Denosumab. While the antibody does not recognize the human IgG (data not shown). Coating antigen: Denosumab, 1 µg/ml. Anti-Denosumab Antibody (5H762) (TMAS-00032) dilution start from 1,000 ng/ml. EC50= 2.921 ng/ml. 2. Anti-Denosumab Antibody (5H762) (TMAS-00032) blocks Denosumab binding with Human RANKL recombinant protein. Coating antigen: Denosumab 1 µg/ml. RANKL final concentration: 12.5 ng/ml. Anti-Denosumab antibody dilutions start from 30 μg/ml. IC50= 0.893 µg/ml. 3. Standard curve of Denosumab Sandwich ELISA. The Denosumab Sandwich ELISA assay is developed by using Anti-Denosumab Antibody (6S249) (TMAS-00030) and Anti-Denosumab Antibody (5H762) (TMAS-00032) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Denosumab Antibody (5H762) (TMAS-00032) was labeled with Biotin. The sensitivity of detecting Denosumab is up to 0.26 ng/ml. |
| 应用 | ELISACompetitive ELISA |
| 推荐剂量 | ELISA: 0.001-0.05 µg/ml; Competitive ELISA: 5-30 µg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 纯化方式 | Protein A affinity column |
| 性状 | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| 缓冲液 | Lyophilized with PBS, pH 7.2, containing 0.02% sodium azide. |
| 研究背景 | Denosumab with trade name Prolia, is an FDA-approved drug for the treatment of patients with osteoporosis, treatment-induced bone loss, metastases to bone, and giant cell tumor of bone. Denosumab is a fully human monoclonal antibody that works as a RANK ligand (RANKL) inhibitor. Denosumab can bind RANKL, preventing RANKL from activating RANK, its receptor on the osteoclast surface. |
| 偶联 | Unconjugated |
| 免疫原 | Denosumab |
| 储存方式 | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |