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Anti-Denosumab Antibody (5H762)

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货号 TMAS-00032

别名 Prolia Xgeva, Prolia, AMG-162, AMG 162

Anti-Denosumab Antibody (5H762) 是一种 Mouse 抗体,靶向 Denosumab。Anti-Denosumab Antibody (5H762) 可用于 ELISA, Competitive ELISA。

Anti-Denosumab Antibody (5H762)

Anti-Denosumab Antibody (5H762)

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Rating icon 还可以
货号 TMAS-00032 别名 Prolia Xgeva, Prolia, AMG-162, AMG 162

Anti-Denosumab Antibody (5H762) 是一种 Mouse 抗体,靶向 Denosumab。Anti-Denosumab Antibody (5H762) 可用于 ELISA, Competitive ELISA。

规格价格库存数量
40 μg
¥ 1,609
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产品介绍


生物活性
产品描述
Anti-Denosumab Antibody (5H762) is a Mouse antibody targeting Denosumab. Anti-Denosumab Antibody (5H762) can be used in ELISA, Competitive ELISA.
别名Prolia Xgeva, Prolia, AMG-162, AMG 162
Ig Type
IgG1,κ
克隆号
5H762
特异性
Denosumab
验证活性
1. ELISA binding of Anti-Denosumab Antibody (5H762) (TMAS-00032) with Denosumab. While the antibody does not recognize the human IgG (data not shown).
Coating antigen: Denosumab, 1 µg/ml.
Anti-Denosumab Antibody (5H762) (TMAS-00032) dilution start from 1,000 ng/ml.
EC50= 2.921 ng/ml.
2. Anti-Denosumab Antibody (5H762) (TMAS-00032) blocks Denosumab binding with Human RANKL recombinant protein.
Coating antigen: Denosumab 1 µg/ml.
RANKL final concentration: 12.5 ng/ml.
Anti-Denosumab antibody dilutions start from 30 μg/ml.
IC50= 0.893 µg/ml.
3. Standard curve of Denosumab Sandwich ELISA. The Denosumab Sandwich ELISA assay is developed by using Anti-Denosumab Antibody (6S249) (TMAS-00030) and Anti-Denosumab Antibody (5H762) (TMAS-00032) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Denosumab Antibody (5H762) (TMAS-00032) was labeled with Biotin. The sensitivity of detecting Denosumab is up to 0.26 ng/ml.
应用ELISACompetitive ELISA
推荐剂量
ELISA: 0.001-0.05 µg/ml; Competitive ELISA: 5-30 µg/ml
抗体种类
Monoclonal
宿主来源Mouse
纯化方式Protein A affinity column
性状Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
缓冲液Lyophilized with PBS, pH 7.2, containing 0.02% sodium azide.
研究背景Denosumab with trade name Prolia, is an FDA-approved drug for the treatment of patients with osteoporosis, treatment-induced bone loss, metastases to bone, and giant cell tumor of bone. Denosumab is a fully human monoclonal antibody that works as a RANK ligand (RANKL) inhibitor. Denosumab can bind RANKL, preventing RANKL from activating RANK, its receptor on the osteoclast surface.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Denosumab
储存&溶解度
储存方式The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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