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Anti-delta 2 Catenin Antibody (8O145) 是一种 Rabbit 抗体,靶向 delta 2 Catenin。Anti-delta 2 Catenin Antibody (8O145) 可用于 WB, ICC/IF, FCM。
Anti-delta 2 Catenin Antibody (8O145) 是一种 Rabbit 抗体,靶向 delta 2 Catenin。Anti-delta 2 Catenin Antibody (8O145) 可用于 WB, ICC/IF, FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 795 | 5日内发货 | |
| 50 μL | ¥ 1,380 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
| 产品描述 | Anti-delta 2 Catenin Antibody (8O145) is a Rabbit antibody targeting delta 2 Catenin. Anti-delta 2 Catenin Antibody (8O145) can be used in WB, ICC/IF, FCM. |
| Ig Type | IgG |
| 克隆号 | 8O145 |
| 反应种属 | Mouse,Rat,Human |
| 验证活性 | 1. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-delta 2 Catenin antibody (TMAB-05071, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. 2. 25 ug total protein per lane of various lysates (see on figure) probed with delta 2 Catenin monoclonal antibody, unconjugated (TMAB-05071) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| 应用 | WBICC/IFFCM |
| 推荐剂量 | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Predominantly expressed in brain. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | Has a critical role in neuronal development, particularly in the formation and/or maintenance of dendritic spines and synapses. Involved in the regulation of canonical Wnt signaling.It probably acts on beta-catenin turnover, facilitating beta-catenin interaction with GSK3B, phosphorylation, ubiquitination and degradation. |
| 免疫原 | A synthesized peptide: mouse delta 2 Catenin |
| 抗原种属 | Mouse |
| 基因名称 | CTNND2 |
| 基因ID | |
| 蛋白名称 | Catenin delta-2 |
| Uniprot ID | |
| 功能 | Functions as a transcriptional activator when bound to ZBTB33 (By similarity). May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules. |
| 分子量 | Theoretical: 135 kDa. Actual: 135 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |