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Anti-DDX4 Antibody (5D840)
还可以产品编号 TMAY-00095
Anti-DDX4 Antibody (5D840) 是一种 Mouse 抗体，靶向 DDX4。Anti-DDX4 Antibody (5D840) 可用于 IHC-P,ICC/IF。
Anti-DDX4 Antibody (5D840)
Anti-DDX4 Antibody (5D840)
还可以产品编号 TMAY-00095
Anti-DDX4 Antibody (5D840) 是一种 Mouse 抗体，靶向 DDX4。Anti-DDX4 Antibody (5D840) 可用于 IHC-P,ICC/IF。
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抗原信息
| 产品描述 | Anti-DDX4 Antibody (5D840) is a Mouse antibody targeting DDX4. Anti-DDX4 Antibody (5D840) can be used in IHC-P,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 5D840 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human DDX4 in human testis with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). 2. Immunofluorescence staining of DDX4 in hESC-H9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-DDX4 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
| 应用 | IHC-P,ICC/IF |
| 推荐剂量 | IHC-P: 1:50-1:200; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human DDX4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | DDX4 (DEAD box polypeptide 4), characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an RNA helicase which is implicated in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX4 is known to be a germ cell-specific protein and is used as a sorting marker of germline stem cells for the production of oocytes. The active vitamin D treatment prohibited the proliferation and invasion of ovarian cancer cells, and the expression level of a germ cell specific marker DEAD (Asp-Glu-Ala-Asp)-box helicase 4 (DDX4), which is overexpressed in ovarian cancer, was downregulated by active vitamin D treatment. Knockdown of DDX4 by siRNA could also suppress the invasive ability of ovarian cancer cells. Therefore, DDX4 may be considered as a diagnostic marker of ovarian cancer, and vitamin D may be a candidate drug for ovarian cancer therapy. That DDX4 is essential for the regulation of germ cell proliferation and differentiation across all three extant mammalian groups-eutherians, marsupials, and monotremes. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the C-terminus of the human DDX4. |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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