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顶部产品介绍抗原信息储存和溶解信息计算器

Anti-DDB1 Antibody (2T437)

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货号 TMAB-05025

Anti-DDB1 Antibody (2T437) 是一种 Mouse 抗体，靶向 DDB1。Anti-DDB1 Antibody (2T437) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-DDB1 Antibody (2T437)

Anti-DDB1 Antibody (2T437)

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Rating icon 还可以
货号 TMAB-05025

Anti-DDB1 Antibody (2T437) 是一种 Mouse 抗体，靶向 DDB1。Anti-DDB1 Antibody (2T437) 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
25 μL
¥ 780
5日内发货
50 μL
¥ 1,395
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-DDB1 Antibody (2T437) is a Mouse antibody targeting DDB1. Anti-DDB1 Antibody (2T437) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG2a, Kappa
克隆号
2T437
反应种属
Human,Mouse,Rat
验证活性
1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, 293, MCF-7, Raw264.7
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 127 kDa
Observed MW: 127 kDa
2. Tissue: Human liver cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-05025
3. Tissue: Rat colon
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-05025
4. Tissue: Mouse liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-05025
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Cytoplasm. Nucleus. Note=Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
构建方式Recombinant Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq].
抗原信息
基因名称
DDB1
基因ID
1642
蛋白名称
DNA damage-binding protein 1
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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