Anti-DcR2 Polyclonal Antibody is a Rabbit antibody targeting DcR2. Anti-DcR2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Blank control: Jurkat cells (blue). Primary Antibody: Rabbit Anti-DcR2 antibody (TMAB-05012), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-05012, 1 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Human MKN45 cell lysates
Lane 2: Human SW480 cell lysates
Lane 3: Human HUVEC cell lysates
Lane 4: Human DU145 cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-DcR2 (TMAB-05012) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human DcR2

Human

TNFRSF10D

Tumor necrosis factor receptor superfamily member 10D

Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway.