购物车
全部删除
您的购物车当前为空
别名 ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase
Anti-DBF4 Antibody (2Q22) 是一种抗体,靶向 DBF4。Anti-DBF4 Antibody (2Q22) 可用于 ELISA,IF,WB。
Anti-DBF4 Antibody (2Q22) 是一种抗体,靶向 DBF4。Anti-DBF4 Antibody (2Q22) 可用于 ELISA,IF,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
| 产品描述 | Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA,IF,WB. |
| 别名 | ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase |
| Ig Type | Rabbit IgG |
| 克隆号 | 2Q22 |
| 反应种属 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate -All lanes: DBF4 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 77, 27 kDa -Observed band size: 72-95 kDa 2. Immunofluorescence staining of A549 cell with TMAH-00332 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 551-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| 应用 | ELISAIFWB |
| 推荐剂量 | WB:1:500-1:2000; IF:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Highly expressed in testis and thymus. Expressed also in most cancer cells lines. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human DBF4 |
| 抗原种属 | Human |
| 基因名称 | DBF4 |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cell biology |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |