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Anti-DBF4 Antibody (2Q22)

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产品编号 TMAH-00332
别名 ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase

Anti-DBF4 Antibody (2Q22) 是一种抗体,靶向 DBF4。Anti-DBF4 Antibody (2Q22) 可用于 ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200。

Anti-DBF4 Antibody (2Q22)

Anti-DBF4 Antibody (2Q22)

Rating icon 还可以
产品编号 TMAH-00332 别名 ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase

Anti-DBF4 Antibody (2Q22) 是一种抗体,靶向 DBF4。Anti-DBF4 Antibody (2Q22) 可用于 ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200。

规格价格库存数量
50 μL
¥ 1,315
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100 μL
¥ 2,190
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产品介绍

生物活性
产品描述
Anti-DBF4 Antibody (2Q22) is an antibody targeting DBF4. Anti-DBF4 Antibody (2Q22) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
别名ZDBF1, Protein DBF4 homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, DBF 4, Chiffon homolog A, ASK, Activator of S phase kinase
Ig Type
Rabbit IgG
克隆号
2Q22
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate
-All lanes: DBF4 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 77, 27 kDa
-Observed band size: 72-95 kDa
2. Immunofluorescence staining of A549 cell with TMAH-00332 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 551-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
应用
ELISA, WB, IF
推荐剂量
WB:1:500-1:2000; IF:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Nucleus.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human DBF4
抗原种属
Human
基因ID
10926
Uniprot ID
研究领域
Epigenetics and Nuclear Signaling, Cell biology
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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