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Anti-Cytokeratin 8 Polyclonal Antibody
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产品编号 TMAB-00534
Anti-Cytokeratin 8 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Cytokeratin 8。Anti-Cytokeratin 8 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,ELISA。
Anti-Cytokeratin 8 Polyclonal Antibody
Anti-Cytokeratin 8 Polyclonal Antibody
还可以产品编号 TMAB-00534
Anti-Cytokeratin 8 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Cytokeratin 8。Anti-Cytokeratin 8 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Cytokeratin 8 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 8. Anti-Cytokeratin 8 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| 交叉反应 | Human (predicted:Mouse,Rat,Chicken) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Human mammary gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]. |
| 免疫原 | KLH conjugated synthetic peptide: human CK8 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cytokeratins |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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