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Anti-Cytokeratin 5 Polyclonal Antibody
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产品编号 TMAB-00532
Anti-Cytokeratin 5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Cytokeratin 5。Anti-Cytokeratin 5 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Cytokeratin 5 Polyclonal Antibody
Anti-Cytokeratin 5 Polyclonal Antibody
还可以产品编号 TMAB-00532
Anti-Cytokeratin 5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Cytokeratin 5。Anti-Cytokeratin 5 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Cytokeratin 5 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 5. Anti-Cytokeratin 5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat bladder tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (TMAB-00532) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (TMAB-00532) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Skin (Mouse) Lysate at 40 μg Lane 2: Uterus (Mouse) Lysate at 40 μg Lane 3: Urinary bladder (Mouse) Lysate at 40 μg Lane 4: Esophagus (Mouse) Lysate at 40 μg Lane 5: Skin (Rat) Lysate at 40 μg Lane 6: Uterus (Rat) Lysate at 40 μg Lane 7: Urinary bladder (Rat) Lysate at 40 μg Lane 8: Esophagus (Rat) Lysate at 40 μg Primary: Anti-Cytokeratin 5 (TMAB-00532) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 60 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (TMAB-00532) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex. |
| 免疫原 | KLH conjugated synthetic peptide: human CK5 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cytokeratins |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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