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Anti-Cytokeratin 5 Antibody (6R761)

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货号 TMAB-04957

Anti-Cytokeratin 5 Antibody (6R761) 是一种 Rabbit 抗体，靶向 Cytokeratin 5。Anti-Cytokeratin 5 Antibody (6R761) 可用于 IF, IHC-Fr, IHC-P, WB。

Anti-Cytokeratin 5 Antibody (6R761)

Anti-Cytokeratin 5 Antibody (6R761)

一键复制产品信息
Rating icon 还可以
货号 TMAB-04957

Anti-Cytokeratin 5 Antibody (6R761) 是一种 Rabbit 抗体，靶向 Cytokeratin 5。Anti-Cytokeratin 5 Antibody (6R761) 可用于 IF, IHC-Fr, IHC-P, WB。

规格价格库存数量
50 μL
¥ 1,385
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

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产品描述
Anti-Cytokeratin 5 Antibody (6R761) is a Rabbit antibody targeting Cytokeratin 5. Anti-Cytokeratin 5 Antibody (6R761) can be used in IF, IHC-Fr, IHC-P, WB.
Ig Type
IgG
克隆号
6R761
反应种属
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded Mouse Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1: 1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1: 1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Laryngeal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Mouse Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5  Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Rat Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5 Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, Goat Anti-Rabbit IgG H&L-BF488), DAPI (blue) was used to stain the cell nuclei.
15. Paraformaldehyde-fixed, paraffin embedded Mouse Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5 Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, Goat Anti-Rabbit IgG H&L-BF488), DAPI (blue) was used to stain the cell nuclei.
16. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 5 Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, Goat Anti-Rabbit IgG H&L-BF488), DAPI (blue) was used to stain the cell nuclei.
17. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 5 Monoclonal Antibody, Unconjugated (TMAB-04957) at 1: 1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
18. 25 ug total protein per lane of various lysates (see on figure) probed with Cytokeratin 5 monoclonal antibody, unconjugated (TMAB-04957) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
19. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 5 Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
20. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 5 Monoclonal Antibody, Unconjugated (TMAB-04957) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
应用IFIHC-FrIHC-PWB
推荐剂量
IF=1:200-2000; IHC-Fr=1:200-2000; IHC-P=1:200-2000; WB=1:500-2000
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.
抗原信息
免疫原
A synthesized peptide: human Cytokeratin 5
抗原种属
Human
基因名称
KRT5
基因ID
3852
蛋白名称
Keratin, type II cytoskeletal 5
Uniprot ID
P13647
化学信息
分子量Theoretical: 64 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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