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Anti-Cytokeratin 13 Antibody (9H545)

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货号 TMAB-04936

Anti-Cytokeratin 13 Antibody (9H545) 是一种 Rabbit 抗体，靶向 Cytokeratin 13。Anti-Cytokeratin 13 Antibody (9H545) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

Anti-Cytokeratin 13 Antibody (9H545)

Anti-Cytokeratin 13 Antibody (9H545)

一键复制产品信息
Rating icon 还可以
货号 TMAB-04936

Anti-Cytokeratin 13 Antibody (9H545) 是一种 Rabbit 抗体，靶向 Cytokeratin 13。Anti-Cytokeratin 13 Antibody (9H545) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

规格价格库存数量
50 μL
¥ 1,395
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

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产品描述
Anti-Cytokeratin 13 Antibody (9H545) is a Rabbit antibody targeting Cytokeratin 13. Anti-Cytokeratin 13 Antibody (9H545) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
克隆号
9H545
反应种属
Human,Mouse
验证活性
1. 25 μg total protein per lane of various lysates (see on figure) probed with Cytokeratin 13 monoclonal antibody, unconjugated (TMAB-04936) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 13 Monoclonal Antibody, Unconjugated (TMAB-04936) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 13 Monoclonal Antibody, Unconjugated (TMAB-04936) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 13 Monoclonal Antibody, Unconjugated (TMAB-04936) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 13 Monoclonal Antibody, Unconjugated (TMAB-04936) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human bladder invasive urothelial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 13 Monoclonal Antibody, Unconjugated (TMAB-04936) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 13 Monoclonal Antibody, Unconjugated (TMAB-04936) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
应用WBIHC-PIHC-FrICC/IFIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
抗体种类
Monoclonal
宿主来源Rabbit
组织特异性Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) . WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008].
抗原信息
免疫原
A synthesized peptide: human Cytokeratin 13
抗原种属
Human
基因名称
KRT13
基因ID
3860
蛋白名称
Keratin, type I cytoskeletal 13
Uniprot ID
P13646
化学信息
分子量Theoretical: 49 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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