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Anti-CYP2R1 Polyclonal Antibody

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货号 TMAB-04891

Anti-CYP2R1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CYP2R1。Anti-CYP2R1 Polyclonal Antibody 可用于 WB,FCM。

Anti-CYP2R1 Polyclonal Antibody

Anti-CYP2R1 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-04891

Anti-CYP2R1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CYP2R1。Anti-CYP2R1 Polyclonal Antibody 可用于 WB,FCM。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-CYP2R1 Polyclonal Antibody is a Rabbit antibody targeting CYP2R1. Anti-CYP2R1 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
反应种属
Human,Mouse (predicted:Rat,Dog,Pig,Horse,Rabbit)
验证活性
1. Sample: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-CYP2R1 (TMAB-04891) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
2. Sample: liver (Human) Cell Lysate at 40 μg
Primary: Anti-CYP2R1 (TMAB-04891) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
3. Sample: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-CYP2R1 (TMAB-04891) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
4. Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-CYP2R1 antibody (TMAB-04891) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
应用WBFCM
推荐剂量
WB: 1:500-2000; FCM: 2μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
组织特异性Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. Defects in CYP2R1 are a cause of 25-hydroxyvitamin D(3) deficiency, also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. First described in patients who had rickets at a young age despite a history of adequate vitamin D intake. The patients sera had low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity and low levels of 25-hydroxyvitamin D.
抗原信息
免疫原
KLH conjugated synthetic peptide: human CYP2R1
抗原种属
Human
基因名称
CYP2R1
基因ID
120227
蛋白名称
Vitamin D 25-hydroxylase
Uniprot ID
Q6VVX0
化学信息
分子量Theoretical: 55 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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