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Anti-CTNNB1 Antibody (9U769)

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Anti-CTNNB1 Antibody (9U769)
产品编号 TMAH-00314
别名 PRO2286, OK/SW-cl.35, NEDSDV, MRD19, EVR7, CTNNB, beta-catenin, armadillo

Anti-CTNNB1 Antibody (9U769) 是一种抗体,靶向 CTNNB1。Anti-CTNNB1 Antibody (9U769) 可用于 ELISA, IHC。

Anti-CTNNB1 Antibody (9U769)

Anti-CTNNB1 Antibody (9U769)

Rating icon 还可以
Anti-CTNNB1 Antibody (9U769)
产品编号 TMAH-00314 别名 PRO2286, OK/SW-cl.35, NEDSDV, MRD19, EVR7, CTNNB, beta-catenin, armadillo

Anti-CTNNB1 Antibody (9U769) 是一种抗体,靶向 CTNNB1。Anti-CTNNB1 Antibody (9U769) 可用于 ELISA, IHC。

规格价格库存数量
50 μL
¥ 1,300
5日内发货
100 μL
¥ 2,195
5日内发货
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产品介绍

生物活性
产品描述
Anti-CTNNB1 Antibody (9U769) is an antibody targeting CTNNB1. Anti-CTNNB1 Antibody (9U769) can be used in ELISA, IHC.
别名PRO2286, OK/SW-cl.35, NEDSDV, MRD19, EVR7, CTNNB, beta-catenin, armadillo
Ig Type
Rabbit IgG
克隆号
9U769
交叉反应
Human
验证活性
1. IHC image of TMAH-00314 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
2. IHC image of TMAH-00314 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用
ELISA, IHC
推荐剂量
IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse. Cytoplasm, cytoskeleton, cilium basal body.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human beta Catenin
抗原种属
Human
基因ID
1499
Uniprot ID
研究领域
Neuroscience, Cancer, Cardiovascular, Signal transduction, Stem cells
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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