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Anti-CTNNB1 Antibody (9U769) 是一种抗体,靶向 CTNNB1。Anti-CTNNB1 Antibody (9U769) 可用于 ELISA, IHC。
Anti-CTNNB1 Antibody (9U769) 是一种抗体,靶向 CTNNB1。Anti-CTNNB1 Antibody (9U769) 可用于 ELISA, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,300 | 5日内发货 | |
100 μL | ¥ 2,195 | 5日内发货 |
产品描述 | Anti-CTNNB1 Antibody (9U769) is an antibody targeting CTNNB1. Anti-CTNNB1 Antibody (9U769) can be used in ELISA, IHC. |
别名 | PRO2286, OK/SW-cl.35, NEDSDV, MRD19, EVR7, CTNNB, beta-catenin, armadillo |
Ig Type | Rabbit IgG |
克隆号 | 9U769 |
交叉反应 | Human |
验证活性 | 1. IHC image of TMAH-00314 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. IHC image of TMAH-00314 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
应用 | ELISA, IHC |
推荐剂量 | IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse. Cytoplasm, cytoskeleton, cilium basal body. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human beta Catenin |
抗原种属 | Human |
基因ID | 1499 |
Uniprot ID | |
研究领域 | Neuroscience, Cancer, Cardiovascular, Signal transduction, Stem cells |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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