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Anti-CTLA4 Polyclonal Antibody

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产品编号 TMAB-00491
别名 IDDM12, ICOS, GSE, GRD4, CTLA4, CELIAC3, CD152, ALPS5

Anti-CTLA4 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CTLA4。Anti-CTLA4 Polyclonal Antibody 可用于 FCM,WB。

Anti-CTLA4 Polyclonal Antibody

Anti-CTLA4 Polyclonal Antibody

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产品编号 TMAB-00491 别名 IDDM12, ICOS, GSE, GRD4, CTLA4, CELIAC3, CD152, ALPS5

Anti-CTLA4 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CTLA4。Anti-CTLA4 Polyclonal Antibody 可用于 FCM,WB。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-CTLA4 Polyclonal Antibody is a Rabbit antibody targeting CTLA4. Anti-CTLA4 Polyclonal Antibody can be used in FCM,WB.
别名IDDM12, ICOS, GSE, GRD4, CTLA4, CELIAC3, CD152, ALPS5
Ig Type
IgG
交叉反应
Human,Mouse,Rat (predicted:Dog,Cow,Rabbit,Sheep)
验证活性
1. Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CTLA4 antibody (TMAB-00491), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
2. Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min)
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
3. Blank control (blue): Mouse Spleen Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Thr325)/FITC Conjugated antibody (TMAB-00491/FITC), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange),used under the same conditions.
4. Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody (TMAB-00491/FITC), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC orange),used under the same conditions.
5. Sample:
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-CTLA4 (TMAB-00491) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 35 kDa
6. Sample:
Liver (Mouse) Cell Lysate at 30 μg
Primary: Anti-CTLA4 (TMAB-00491) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 35 kDa
7. Sample:
Liver (Rat) Cell Lysate at 30 μg
Primary: Anti-CTLA4 (TMAB-00491) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 35 kDa
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用FCMWB
推荐剂量
WB: 1:500-2000; FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation.
组织特异性Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human CTLA-4/CD152
抗原种属
Human
基因名称
CTLA4
基因ID
蛋白名称
Cytotoxic T-lymphocyte protein 4
Uniprot ID
研究领域
Regulatory T Cells,CD,Cytotoxic Cells,Autoimmune,SARS Coronavirus,T Lymphocytic Lineage
功能
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
化学信息
分子量Theoretical: 21 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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