Anti-CTLA4 Antibody (7G99) is a Mouse antibody targeting CTLA4. Anti-CTLA4 Antibody (7G99) can be used in ELISA, WB, IHC.

IgG1

7G99

Human

1. Western Blot
-Positive WB detected in: CEM whole cell lysate, K562 whole cell lysate, Raji whole cell lysate
-All lanes: CTLA4 antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 25, 7, 9, 20 KDa
-Observed band size: 34 KDa
-Exposure time:5min
2. IHC image of TMAH-00310 diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00310 diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
4. IHC image of TMAH-00310 diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

Monoclonal

Unconjugated

Recombinant Protein: Human Cytotoxic T-lymphocyte Protein 4 Protein (37-162AA)

Human

1493

Immunology