- 全部删除
- 您的购物车当前为空
Anti-CRY1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CRY1。Anti-CRY1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-CRY1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CRY1。Anti-CRY1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-CRY1 Polyclonal Antibody is a Rabbit antibody targeting CRY1. Anti-CRY1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep) |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cryptochrome 1) Polyclonal Antibody, Unconjugated (TMAB-00484) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Cryptochrome-1 Polyclonal Antibody, Unconjugated (TMAB-00484) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human 293T cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-Cryptochrome 1 (TMAB-00484) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 70 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Circadian clocks are biological timepieces that regulate hormonal rhythms, sleep cycles and feeding behaviors. These rhythms are generated in the superchiasmatic nucleus (SCN), a cell-autonomous circadian oscillator located within the brain that is synchronized with the environment by light. A number of transcription factors, including Clock and BMAL1, are molecular components of the SCN that induce the expression of proteins involved in light/dark cycle entrainment, which include Per1 and Per2. Tim, for timeless, generates a negative feedback loop that regulates the activity of Clock by suppressing the expression of Clock target genes. Tim forms heterodimers with Per1 and Per2 that bind Clock and block the activation of Clock-BMAL1 dimers to repress Per gene expression. Additionally, the CRY proteins, which are cryptochrome photoreceptors for the circadian clock, function as light-independent inhibitors of the circadian clock. CRY1 and CRY2 negatively regulate SCN components by associating with the activators Clock-BMAL1, and also with the various feedback inhibitors Per1, Per2 and Tim. |
免疫原 | KLH conjugated synthetic peptide: human Cryptochrome 1 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Blood Pressure regulation,Catecholamines,Circadian Rhythm,Receptors,More GPCR,Visual system |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
评论内容