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Anti-CRY1 Polyclonal Antibody

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Anti-CRY1 Polyclonal Antibody
产品编号 TMAB-00484

Anti-CRY1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CRY1。Anti-CRY1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-CRY1 Polyclonal Antibody

Anti-CRY1 Polyclonal Antibody

Rating icon 还可以
Anti-CRY1 Polyclonal Antibody
产品编号 TMAB-00484

Anti-CRY1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CRY1。Anti-CRY1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-CRY1 Polyclonal Antibody is a Rabbit antibody targeting CRY1. Anti-CRY1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cryptochrome 1) Polyclonal Antibody, Unconjugated (TMAB-00484) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Cryptochrome-1 Polyclonal Antibody, Unconjugated (TMAB-00484) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human 293T cell lysates
Lane 3: Human MCF-7 cell lysates
Primary: Anti-Cryptochrome 1 (TMAB-00484) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 70 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Circadian clocks are biological timepieces that regulate hormonal rhythms, sleep cycles and feeding behaviors. These rhythms are generated in the superchiasmatic nucleus (SCN), a cell-autonomous circadian oscillator located within the brain that is synchronized with the environment by light. A number of transcription factors, including Clock and BMAL1, are molecular components of the SCN that induce the expression of proteins involved in light/dark cycle entrainment, which include Per1 and Per2. Tim, for timeless, generates a negative feedback loop that regulates the activity of Clock by suppressing the expression of Clock target genes. Tim forms heterodimers with Per1 and Per2 that bind Clock and block the activation of Clock-BMAL1 dimers to repress Per gene expression. Additionally, the CRY proteins, which are cryptochrome photoreceptors for the circadian clock, function as light-independent inhibitors of the circadian clock. CRY1 and CRY2 negatively regulate SCN components by associating with the activators Clock-BMAL1, and also with the various feedback inhibitors Per1, Per2 and Tim.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Cryptochrome 1
抗原种属
Human
基因ID
Uniprot ID
研究领域
Blood Pressure regulation,Catecholamines,Circadian Rhythm,Receptors,More GPCR,Visual system
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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