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Anti-CRISP11 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CRISP11。Anti-CRISP11 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-CRISP11 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 CRISP11。Anti-CRISP11 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
| 产品描述 | Anti-CRISP11 Polyclonal Antibody is a Rabbit antibody targeting CRISP11. Anti-CRISP11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 反应种属 | Mouse,Rat (predicted:Human,Dog,Pig,Cow,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRISP11) Polyclonal Antibody, Unconjugated (TMAB-04675) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: pancreas (Mouse) Lysate at 40 μg Primary: Anti-CRISP11 (TMAB-04675) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | Cysteine-rich secretory proteins (CRISPs) represent a family of evolutionarily conserved proteins that may play a role in the innate immune system and are transcriptionally regulated by androgens in several tissues. CRISP proteins are highly expressed in the mammalian reproductive tract and in the venom secretory ducts of some reptiles. CRISP-11 (cysteine-rich secretory protein 11), also known as cysteine-rich secretory protein LCCL domain-containing 2 (CRISPLD2 or LCRISP2), is a 497 amino acid protein containing 2 LCCL domains, which are thought to function as autonomous folding domains used to construct modular proteins through exon shuffling. Serum concentrations of CRISP-11 have been shown to be an indicator of a patient’s exposure to lipopolysaccahride (LPS), the immunostimulatory component of Gram-negative bacteria, and can determine one’s sensitivity to it. |
| 免疫原 | KLH conjugated synthetic peptide: human CRISP11 |
| 抗原种属 | Human |
| 基因名称 | CRISPLD2 |
| 基因ID | |
| 蛋白名称 | Cysteine-rich secretory protein LCCL domain-containing 2 |
| Uniprot ID | |
| 功能 | Promotes matrix assembly. |
| 分子量 | Theoretical: 54 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |