Anti-CRHR1 Polyclonal Antibody is a Rabbit antibody targeting CRHR1. Anti-CRHR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human)

1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Cerebellum (Mouse) Lysate at 40 μg
Lane 3: Testis (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Lane 5: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-CRHR1 (TMAB-00481) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kDa
Observed band size: 48 kDa
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (TMAB-00481) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (TMAB-00481) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated (TMAB-00481) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CRHR1

Human

Cancer,Obesity,Hypothalamic pituitary adrenal axis,Metabolism,Corticoid