Anti-CREB1 Antibody (9J76) is an antibody targeting CREB1. Anti-CREB1 Antibody (9J76) can be used in ELISA, WB, FC.

Rabbit IgG

9J76

Human, Mouse, Rat

1. Western Blot
-Positive WB detected in: K562 whole cell lysate, THP-1 whole cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate,
-All lanes: CREB antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 36 kDa
-Observed band size: 36 kDa
2. Overlay Peak curve showing Jurkat cells stained with TMAH-00298 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FC

WB:1:500-1:2000; FC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human CREB1

Human

1385

Epigenetics and Nuclear Signaling, Immunology