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Anti-CREB1 Antibody (3J147)
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产品编号 TMAB-00478
Anti-CREB1 Antibody (3J147) 是一种 Mouse 抗体，靶向 CREB1。Anti-CREB1 Antibody (3J147) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-CREB1 Antibody (3J147)
Anti-CREB1 Antibody (3J147)
还可以产品编号 TMAB-00478
Anti-CREB1 Antibody (3J147) 是一种 Mouse 抗体，靶向 CREB1。Anti-CREB1 Antibody (3J147) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-CREB1 Antibody (3J147) is a Mouse antibody targeting CREB1. Anti-CREB1 Antibody (3J147) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 3J147 |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00478) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Jurkat (Human) Cell Lysate at 30 μg Huvec (Human) Cell Lysate at 30 μg U2os (Human) Cell Lysate at 30 μg Primary: Anti-CREB-1 (TMAB-00478) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 43 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00478) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of TMAB-00478) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Sample: NIH/3T3 Cell (Mouse) Lysate at 40 μg Primary: Anti-CREB-1 (TMAB-00478) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 45 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF-2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator. |
| 免疫原 | Recombinant Protein: human CREB-1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | HLH / Leucine Zipper,Transcription Factors,TLR Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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