还可以
Anti-CRB2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CRB2。Anti-CRB2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
还可以Anti-CRB2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CRB2。Anti-CRB2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
| 产品描述 | Anti-CRB2 Polyclonal Antibody is a Rabbit antibody targeting CRB2. Anti-CRB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| 别名 | RP11-230L22.2, Crumbs-like protein 2, Crumbs homolog 2(Drosophila), Crumbs homolog 2, CRUM2, Crb2 |
| Ig Type | IgG |
| 交叉反应 | Mouse,Rat (predicted:Human) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRB2) Polyclonal Antibody, Unconjugated (TMAB-00476) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Eye (Rat) Lysate at 40 μg Primary: Anti-CRB2 (TMAB-00476) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100/123/131 kDa Observed band size: 135 kDa 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-CRB2 (TMAB-00476) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100，123，131 kDa Observed band size: 100,131 kDa 4. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (TMAB-00476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (TMAB-00476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted and Cell membrane. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | CRB2 is a 1,285 amino acid protein that contains three laminin G-like domains and 15 EGF-like domains and exists as multiple alternatively spliced isoforms that are either secreted or membrane bound. Expressed in kidney and retina, as well as in fetal eye and brain, CRB2 is thought to play a role in the morphogenesis of polarized cells and may be involved in DNA repair. Defects in the gene encoding CRB2 are associated with the pathogenesis of autosomal recessive retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA), the former of which refers to a group of diseases that lead to the degeneration of retinal photoreceptor cells, ultimately resulting in a loss of vision. The gene encoding CRB2 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome. |
| 免疫原 | KLH conjugated synthetic peptide: human CRB2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Other Antibodies |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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