Anti-CRB2 Polyclonal Antibody is a Rabbit antibody targeting CRB2. Anti-CRB2 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat (predicted:Human)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRB2) Polyclonal Antibody, Unconjugated (TMAB-00476) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Eye (Rat) Lysate at 40 μg
Primary: Anti-CRB2 (TMAB-00476) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100/123/131 kDa
Observed band size: 135 kDa
3. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-CRB2 (TMAB-00476) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100，123，131 kDa
Observed band size: 100,131 kDa
4. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (TMAB-00476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (TMAB-00476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CRB2

Human

CRB2

Protein crumbs homolog 2

Other Antibodies

May play a role in polarized cells morphogenesis.