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Anti-COX5B Antibody (3I109)

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Anti-COX5B Antibody (3I109)
产品编号 TMAY-02788
别名 cytochrome c oxidase subunit Vb, COXVB

Anti-COX5B Antibody (3I109) 是一种 Rabbit 抗体，靶向 COX5B。Anti-COX5B Antibody (3I109) 可用于 WB,ELISA,IP。

Anti-COX5B Antibody (3I109)

Anti-COX5B Antibody (3I109)

Rating icon 还可以
Anti-COX5B Antibody (3I109)
产品编号 TMAY-02788 别名 cytochrome c oxidase subunit Vb, COXVB

Anti-COX5B Antibody (3I109) 是一种 Rabbit 抗体，靶向 COX5B。Anti-COX5B Antibody (3I109) 可用于 WB,ELISA,IP。

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100 μL
¥ 2,289
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生物活性
产品描述
Anti-COX5B Antibody (3I109) is a Rabbit antibody targeting COX5B. Anti-COX5B Antibody (3I109) can be used in WB,ELISA,IP.
别名cytochrome c oxidase subunit Vb, COXVB
Ig Type
Rabbit IgG
克隆号
3I109
交叉反应
Human
验证活性
1. Anti-COX5B rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: A549 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:14 kDa.
-Observed band size:15 kDa.
2. COX5B was immunoprecipitated using:
-Lane A:0.5 mg A549 Whole Cell Lysate.
-Lane B:0.5 mg Hela Whole Cell Lysate.
-Lane C:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-COX5B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-COX5B rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 14 kDa.
-Observed band size: 14 kDa
应用
WB
ELISA
IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human COX5B (rh COX5B; TMPY-03511; P10606; Ala32-His129).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human COX5B Protein (TMPY-03511)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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