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Anti-Corin Polyclonal Antibody

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货号 TMAB-04588

Anti-Corin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Corin。Anti-Corin Polyclonal Antibody 可用于 FCM。

Anti-Corin Polyclonal Antibody

Anti-Corin Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-04588

Anti-Corin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Corin。Anti-Corin Polyclonal Antibody 可用于 FCM。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍


生物活性
产品描述
Anti-Corin Polyclonal Antibody is a Rabbit antibody targeting Corin. Anti-Corin Polyclonal Antibody can be used in FCM.
Ig Type
IgG
反应种属
Human (predicted:Horse,Pig,Dog,Rat,Mouse)
验证活性
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Corin antibody (TMAB-04588)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
应用FCM
推荐剂量
FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Single-pass type II membrane protein. Isoform 2: Cell membrane; Single-pass type II membrane protein. Note=Less efficiently targeted to the cell membrane compared to isoform 1. Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment: Secreted. Note=Soluble form produced following cleavage by ADAM10. Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment: Secreted. Note=Soluble form produced following autocatalytic cleavage. Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment: Secreted. Note=Soluble form produced following autocatalytic cleavage.
组织特异性Highly expressed in heart. Expressed in heart myocytes. Also expressed in pregnant uterus.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Corin was first described as a trypsin-like serine proteinase in the heart which processed pro-atrial natriuretic peptide into the active form ANP. Corin cleaves pro-ANP at Arg98/Ser-99, and blocking Corin message inhibits pro-ANP processing. Null mutant mice lacking Corin develop hypertension, and a series of missense mutations have been identified in humans with high blood pressure and hypertension. Corin is a member of the type-II transmembrane serine proteinases, such as Hepsin, Epitheliasin, Matriptase and enteropeptidase. The Corin domain structure includes a cytoplasmic domain, a transmembrane domain, a spacer region, a frizzled-like domain, 5 LDL Receptor-like domains, another frizzled-like domain, 3 more LDLR-like repeats, a SRCR (Scavenger Receptor-Cysteine Rich) domain (also called macrophage scavenger-like domain), and finally the trypsin-like serine proteinase domain. The complex domain structure puts Corin into the “mosaic” TMPRSS family, so named because of the wide variety of domains.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Corin
抗原种属
Human
基因名称
CORIN
基因ID
蛋白名称
Atrial natriuretic peptide-converting enzyme
Uniprot ID
功能
Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide.Isoform 2: has weaker endopeptidase activity compared to isoform 1.
化学信息
分子量Theoretical: 115 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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