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Anti-Collagen XVII/COL17A1 Antibody (3R637)
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产品编号 TMAB-00464
Anti-Collagen XVII/COL17A1 Antibody (3R637) 是一种 Rabbit 抗体，靶向 Collagen XVII/COL17A1。Anti-Collagen XVII/COL17A1 Antibody (3R637) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Collagen XVII/COL17A1 Antibody (3R637)
Anti-Collagen XVII/COL17A1 Antibody (3R637)
还可以产品编号 TMAB-00464
Anti-Collagen XVII/COL17A1 Antibody (3R637) 是一种 Rabbit 抗体，靶向 Collagen XVII/COL17A1。Anti-Collagen XVII/COL17A1 Antibody (3R637) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 780 | 5日内发货 | |
| 50 μL | ¥ 1,385 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Collagen XVII/COL17A1 Antibody (3R637) is a Rabbit antibody targeting Collagen XVII/COL17A1. Anti-Collagen XVII/COL17A1 Antibody (3R637) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 3R637 |
| 交叉反应 | Rat,Mouse,Human |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (TMAB-00464) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (TMAB-00464) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (TMAB-00464) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. 25 μg total protein per Lane of various lysates probed with Collagen XVII monoclonal antibody, unconjugated (TMAB-00464) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen XVII Monoclonal Antibody, Unconjugated (TMAB-00464) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen XVII Monoclonal Antibody, Unconjugated (TMAB-00464) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome. 120 kDa linear IgA disease antigen and 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008] |
| 免疫原 | A synthesized peptide: human Collagen XVII alpha 1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Collagen |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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