Anti-Collagen II/COL2A1 Polyclonal Antibody 2 is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
293t (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
LOVO (Human) Cell Lysate at 40 μg
MCF-7 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-Collagen II (TMAB-00458) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
2. Sample:
MG63 (Human) Cell Lysate at 30 μg
Primary: Anti-Collagen II (TMAB-00458) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
3. Paraformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (TMAB-00458) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00458) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Sample: bone (Mouse) Lysate at 40 μg
Primary: Anti-Collagen Ii (TMAB-00458) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 134 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Collagen II

Human

COL2A1

Procollagen Type II N-Terminal Propeptide

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.