Anti-Collagen II/COL2A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rabbit (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,GuineaPig)

1. Sample:
293T (human) cell Lysate at 40 μg
A549 (human) cell Lysate at 40 μg
MCF-7 (human) cell Lysate at 40 μg
HepG2 (human) cell Lysate at 40 μg
Placenta (mouse) Lysate at 40 μg
MG63 (human) cell Lysate at 40 μg
Primary: Anti-Collagen II (TMAB-00456) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 120 kDa
2. Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00456) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Collagen II

Human