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Anti-Coagulation factor IX/F9 Antibody (1B255)

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产品编号 TMAY-01880

Anti-Coagulation factor IX/F9 Antibody (1B255) 是一种 Rabbit 抗体,靶向 Coagulation factor IX/F9。Anti-Coagulation factor IX/F9 Antibody (1B255) 可用于 WB,ELISA,IP。

Anti-Coagulation factor IX/F9 Antibody (1B255)

Anti-Coagulation factor IX/F9 Antibody (1B255)

Rating icon 还可以
产品编号 TMAY-01880

Anti-Coagulation factor IX/F9 Antibody (1B255) 是一种 Rabbit 抗体,靶向 Coagulation factor IX/F9。Anti-Coagulation factor IX/F9 Antibody (1B255) 可用于 WB,ELISA,IP。

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产品介绍

生物活性
产品描述
Anti-Coagulation factor IX/F9 Antibody (1B255) is a Rabbit antibody targeting Coagulation factor IX/F9. Anti-Coagulation factor IX/F9 Antibody (1B255) can be used in WB,ELISA,IP.
Ig Type
Monoclonal Rabbit IgG
克隆号
1B255
交叉反应
Human
验证活性
1. Anti-F9 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: A431 Whole Cell Lysate.

-Lane B: Jurkat Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:52 kDa.

-Observed band size:55 kDa.

2. F9 was immunoprecipitated using:

-Lane A:0.5 mg A431 Whole Cell Lysate.

-Lane B:0.5 mg Jurkat Whole Cell Lysate

-0.5 µL anti-F9 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

-Primary antibody:

-Anti-F9 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size: 55 kDa.

-Observed band size:55 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.1-0.5 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Coagulation Factor IX / FIX / F9 (rh Coagulation Factor IX / FIX / F9; TMPY-02215; AAB59620.1; Met 1-Thr 461).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human Coagulation Factor IX / FIX / F9 protein (TMPY-02215)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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