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Anti-Coagulation factor IX/F9 Antibody (1B255) 是一种 Rabbit 抗体,靶向 Coagulation factor IX/F9。Anti-Coagulation factor IX/F9 Antibody (1B255) 可用于 WB,ELISA,IP。
Anti-Coagulation factor IX/F9 Antibody (1B255) 是一种 Rabbit 抗体,靶向 Coagulation factor IX/F9。Anti-Coagulation factor IX/F9 Antibody (1B255) 可用于 WB,ELISA,IP。
产品描述 | Anti-Coagulation factor IX/F9 Antibody (1B255) is a Rabbit antibody targeting Coagulation factor IX/F9. Anti-Coagulation factor IX/F9 Antibody (1B255) can be used in WB,ELISA,IP. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 1B255 |
交叉反应 | Human |
验证活性 | 1. Anti-F9 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell Lysate. -Lane B: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:52 kDa. -Observed band size:55 kDa. 2. F9 was immunoprecipitated using: -Lane A:0.5 mg A431 Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-F9 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-F9 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 55 kDa. -Observed band size:55 kDa |
应用 | WB,ELISA,IP |
推荐剂量 | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.1-0.5 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Coagulation Factor IX / FIX / F9 (rh Coagulation Factor IX / FIX / F9; TMPY-02215; AAB59620.1; Met 1-Thr 461). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease. |
偶联 | Unconjugated |
免疫原 | Recombinant Human Coagulation Factor IX / FIX / F9 protein (TMPY-02215) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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