Anti-CNTF Polyclonal Antibody is a Rabbit antibody targeting CNTF. Anti-CNTF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Rat,Rabbit (predicted:Human,Mouse,Pig)

1. Tissue/cell: rat spinal cord tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Tissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CNTF

Human

CNTF

Ciliary neurotrophic factor

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.