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Anti-CLEC9A Polyclonal Antibody

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Anti-CLEC9A Polyclonal Antibody
产品编号 TMAB-00450

Anti-CLEC9A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CLEC9A。Anti-CLEC9A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-CLEC9A Polyclonal Antibody

Anti-CLEC9A Polyclonal Antibody

Rating icon 还可以
Anti-CLEC9A Polyclonal Antibody
产品编号 TMAB-00450

Anti-CLEC9A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CLEC9A。Anti-CLEC9A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-CLEC9A Polyclonal Antibody is a Rabbit antibody targeting CLEC9A. Anti-CLEC9A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Mouse,Rat (predicted:Human)
验证活性
1. Tissue/cell: rat injured brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CLEC9A Polyclonal Antibody, Unconjugated (TMAB-00450) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-CLEC9A (TMAB-00450) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa
应用
WB
IHC-P
IHC-Fr
IF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Membrane.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A (C-type lectin domain family 9 member A), also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin-like receptor (CTLR) that acts as an activation receptor. The gene encoding CLEC-9A maps to human chromosome 12p13.2 and mouse chromosome 6 F3.
抗原信息
免疫原
KLH conjugated synthetic peptide: human CLEC9A
抗原种属
Human
基因ID
283420
研究领域
Myeloid Cells,Surface Molecules
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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