Anti-CKMT Polyclonal Antibody is a Rabbit antibody targeting CKMT. Anti-CKMT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)

1. Sample: Small intestine (Mouse) Lysate at 40 μg
Primary: Anti-CKMT (TMAB-04410) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CKMT/Creatine kinase MT Polyclonal Antibody, Unconjugated (TMAB-04410) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-CKMT antibody (TMAB-04410)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. Sample:
Lane 1: Mouse Large intestine tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Large intestine tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-CKMT (TMAB-04410) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CKMT

Human

CKMT1A/B

Creatine kinase U-type, mitochondrial