Anti-CK10/Cytokeratin 10 Antibody (1G407) is a Rabbit antibody targeting CK10/Cytokeratin 10. Anti-CK10/Cytokeratin 10 Antibody (1G407) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1G407

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
6. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
7. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
8. 25 μg total protein per lane of various lysates (see on figure) probed with CK10/Cytokeratin 10 monoclonal antibody, unconjugated (TMAB-04382) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
9. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:1000-10000; IHC-P: 1:1000-5000; IHC-Fr: 1:1000-5000; IF: 1:1000-5000

Monoclonal

A synthesized peptide: human Cytokeratin 10

Human

KRT10

Keratin, type I cytoskeletal 10