Anti-CHRNA7 Polyclonal Antibody is a Rabbit antibody targeting CHRNA7. Anti-CHRNA7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken)

1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated (TMAB-00430) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated (TMAB-00430) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
3. Sample:
Lane 1: Human SH-SY5Y cell Lysates
Lane 2: Human U-87 MG cell Lysates
Lane 3: Human U251 cell Lysates
Primary: Anti-CHRNA7 (TMAB-00430) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
4. Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Adrenal glands (Rat) Lysate at 40 μg
Lane 4: Adrenal glands (Mouse) Lysate at 40 μg
Lane 5: Placenta (Mouse) Lysate at 40 μg
Lane 6: Lung (Mouse) Lysate at 40 μg
Lane 7: Testis (Rat) Lysate at 40 μg
Lane 8: HepG2 (Human) Cell Lysate at 30 μg
Lane 9: SH-SY5Y (Human) Cell Lysate at 30 μg
Primary: Anti-CHRNA7 (TMAB-00430) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56/50 kDa
Observed band size: 48 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CHRNA7

Human

nAch Receptors,Hypoxia,Response to hypoxia