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Anti-CHRM3 Polyclonal Antibody

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Anti-CHRM3 Polyclonal Antibody
产品编号 TMAB-00429

Anti-CHRM3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CHRM3。Anti-CHRM3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-CHRM3 Polyclonal Antibody

Anti-CHRM3 Polyclonal Antibody

Rating icon 还可以
Anti-CHRM3 Polyclonal Antibody
产品编号 TMAB-00429

Anti-CHRM3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CHRM3。Anti-CHRM3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-CHRM3 Polyclonal Antibody is a Rabbit antibody targeting CHRM3. Anti-CHRM3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human (predicted:Mouse,Rat)
验证活性
1. Sample: Mcf-7 Cell Lysate at 40 μg
Primary: Anti-Muscarinic Acetylcholine receptor (TMAB-00429) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ChRM3/Acetylcholine receptor (M3) Polyclonal Antibody, Unconjugated (TMAB-00429) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining
应用
WB
IHC-P
IHC-Fr
IF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq].
抗原信息
免疫原
KLH conjugated synthetic peptide: human ChRM3
抗原种属
Human
基因ID
1131
Uniprot ID
P20309
研究领域
Muscarinic Receptors,Acetylcholine
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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