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Anti-CHRM3 Antibody (4D146) 是一种抗体,靶向 CHRM3。Anti-CHRM3 Antibody (4D146) 可用于 ELISA, WB, IHC。
Anti-CHRM3 Antibody (4D146) 是一种抗体,靶向 CHRM3。Anti-CHRM3 Antibody (4D146) 可用于 ELISA, WB, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,305 | 5日内发货 | |
100 μL | ¥ 2,195 | 5日内发货 |
产品描述 | Anti-CHRM3 Antibody (4D146) is an antibody targeting CHRM3. Anti-CHRM3 Antibody (4D146) can be used in ELISA, WB, IHC. |
Ig Type | Rabbit IgG |
克隆号 | 4D146 |
交叉反应 | Human |
验证活性 | 1. IHC image of TMAH-00272 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody 2. IHC image of TMAH-00272 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody |
应用 | |
推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human Muscarinic Acetylcholine Receptor M3 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Neuroscience |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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