Anti-CDK2 Antibody (9U57)

Name: Anti-CDK2 Antibody (9U57)
Brand: TargetMol
SKU: TMAH-00252
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00252

别名 p33(CDK2), cyclin-dependent kinase 2, CDKN2

Anti-CDK2 Antibody (9U57) 是一种抗体，靶向 CDK2。Anti-CDK2 Antibody (9U57) 可用于 ELISA, WB, IHC。

Anti-CDK2 Antibody (9U57)

还可以

产品编号 TMAH-00252 别名 p33(CDK2), cyclin-dependent kinase 2, CDKN2

Anti-CDK2 Antibody (9U57) 是一种抗体，靶向 CDK2。Anti-CDK2 Antibody (9U57) 可用于 ELISA, WB, IHC。

规格	价格	库存	数量
50 μL	¥ 1,315	5日内发货
100 μL	¥ 2,195	5日内发货

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产品描述	Anti-CDK2 Antibody (9U57) is an antibody targeting CDK2. Anti-CDK2 Antibody (9U57) can be used in ELISA, WB, IHC.
别名	p33(CDK2), cyclin-dependent kinase 2, CDKN2
Ig Type	Rabbit IgG
克隆号	9U57
交叉反应	Human, Mouse
验证活性	1. Western Blot -Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate -All lanes: CDK2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34, 31 kDa -Observed band size: 34 kDa 2. IHC image of TMAH-00252 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用	ELISA WB IHC
推荐剂量	WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类	Monoclonal
亚细胞定位	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1. Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability. Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	A synthetic peptide: Human Cdk2
抗原种属	Human
基因名称	CDK2
基因ID	1017
Uniprot ID	P24941
研究领域	Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Cell biology, Signal transduction