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Anti-CDK2 Antibody (9U57)
还可以产品编号 TMAH-00252
别名 p33(CDK2), cyclin-dependent kinase 2, CDKN2
Anti-CDK2 Antibody (9U57) 是一种抗体，靶向 CDK2。Anti-CDK2 Antibody (9U57) 可用于 ELISA, WB, IHC。
Anti-CDK2 Antibody (9U57)
Anti-CDK2 Antibody (9U57)
还可以产品编号 TMAH-00252 别名 p33(CDK2), cyclin-dependent kinase 2, CDKN2
Anti-CDK2 Antibody (9U57) 是一种抗体，靶向 CDK2。Anti-CDK2 Antibody (9U57) 可用于 ELISA, WB, IHC。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CDK2 Antibody (9U57) is an antibody targeting CDK2. Anti-CDK2 Antibody (9U57) can be used in ELISA, WB, IHC. |
| 别名 | p33(CDK2), cyclin-dependent kinase 2, CDKN2 |
| Ig Type | Rabbit IgG |
| 克隆号 | 9U57 |
| 交叉反应 | Human, Mouse |
| 验证活性 | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate -All lanes: CDK2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34, 31 kDa -Observed band size: 34 kDa 2. IHC image of TMAH-00252 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3). |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome an... |
| 偶联 | Unconjugated |
| 免疫原 | A synthesized peptide derived from human Cdk2 |
| 抗原种属 | Human |
| 基因ID | 1017 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Cell biology, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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