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顶部产品介绍偶联与修饰抗原信息储存和溶解信息计算器

Anti-CDC42 Antibody (3V386)

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货号 TMAH-00240

别名 G25K, cell division cycle 42, CDC42Hs

Anti-CDC42 Antibody (3V386) 是一种抗体，靶向 CDC42。Anti-CDC42 Antibody (3V386) 可用于 ELISA,IHC,WB。

Anti-CDC42 Antibody (3V386)

Anti-CDC42 Antibody (3V386)

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货号 TMAH-00240 别名 G25K, cell division cycle 42, CDC42Hs

Anti-CDC42 Antibody (3V386) 是一种抗体，靶向 CDC42。Anti-CDC42 Antibody (3V386) 可用于 ELISA,IHC,WB。

规格价格库存数量
50 μL
¥ 1,315
5日内发货
100 μL
¥ 2,195
5日内发货
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产品介绍

生物活性
产品描述
Anti-CDC42 Antibody (3V386) is an antibody targeting CDC42. Anti-CDC42 Antibody (3V386) can be used in ELISA,IHC,WB.
别名G25K, cell division cycle 42, CDC42Hs
Ig Type
Rabbit IgG
克隆号
3V386
反应种属
Human, Mouse
验证活性
1. Western Blot
-Positive WB detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse Brain whole cell lysate
-All lanes: CDC42 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 22, 22 kDa
-Observed band size: 24 kDa
2. IHC image of TMAH-00240 diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用ELISAIHCWB
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cell projection, dendrite.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human CDC42
抗原种属
Human
基因名称
CDC42
基因ID
998
Uniprot ID
P60953
研究领域
Epigenetics and Nuclear Signaling, Cancer, Cell biology, Signal transduction
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | -20°C for 1 year

计算器

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