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Anti-CD84 Antibody (9Z499)
还可以产品编号 TMAY-00255
Anti-CD84 Antibody (9Z499) 是一种 Rabbit 抗体，靶向 CD84。Anti-CD84 Antibody (9Z499) 可用于 WB,ELISA,IP。
Anti-CD84 Antibody (9Z499)
Anti-CD84 Antibody (9Z499)
还可以产品编号 TMAY-00255
Anti-CD84 Antibody (9Z499) 是一种 Rabbit 抗体，靶向 CD84。Anti-CD84 Antibody (9Z499) 可用于 WB,ELISA,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CD84 Antibody (9Z499) is a Rabbit antibody targeting CD84. Anti-CD84 Antibody (9Z499) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 9Z499 |
| 交叉反应 | Human |
| 验证活性 | 1. CD84 was immunoprecipitated using: -Lane A:0.5 mg Raw264.7 Whole Cell Lysate -0.5 µL anti-CD84 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CD84 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 62 kDa. -Observed band size: 62 kDa. 2. Anti-CD84 rabbit monoclonal antibody at 1:500 dilution. -Lane A: RAW264.7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:39 kDa. -Observed band size:39 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD84 extracellular domain (rh CD84; TMPY-00715; Met 1-Gly 225; NP_003865.1). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human CD84 protein (TMPY-00715) |
| 抗原种属 | Human |
| 研究领域 | ITIM/ITAM Immunoreceptors and Related Molecules |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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