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Anti-CD83 Polyclonal Antibody

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货号 TMAB-04081

Anti-CD83 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD83。Anti-CD83 Polyclonal Antibody 可用于 FCM。

Anti-CD83 Polyclonal Antibody

Anti-CD83 Polyclonal Antibody

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货号 TMAB-04081

Anti-CD83 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD83。Anti-CD83 Polyclonal Antibody 可用于 FCM。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-CD83 Polyclonal Antibody is a Rabbit antibody targeting CD83. Anti-CD83 Polyclonal Antibody can be used in FCM.
Ig Type
IgG
反应种属
Human (predicted:Mouse,Rat)
验证活性
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CD83 antibody (TMAB-04081)
Dilution: 3 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
应用FCM
推荐剂量
FCM: 3μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Membrane; Single-pass type I membrane protein.
组织特异性Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The CD83 antigen is a 186 amino acid single chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V type immunoglobulin-like single domain, a transmembrane region, and a short, 40 amino acid cytoplasmic tail. CD83 antigen undergoes extensive post translational glycosylation, as the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immunophenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also coexpress T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36) and cytokine receptors, as well as other cell surface molecules.
抗原信息
免疫原
KLH conjugated synthetic peptide: human CD83
抗原种属
Human
基因名称
CD83
基因ID
9308
蛋白名称
CD83 antigen
Uniprot ID
Q01151
功能
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
化学信息
分子量Theoretical: 21 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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