Anti-CD82 Antibody (9T113) is a Rabbit antibody targeting CD82. Anti-CD82 Antibody (9T113) can be used in FCM, ICC/IF.

IgG

9T113

Human (predicted:Rat)

1. Western blot analysis of Jurkat cell lysate. Using CD82 (TMAB-04079) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (CD82) monoclonal Antibody, unconjugated (TMAB-04079) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The Raji (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD82 antibody (TMAB-04079, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:100-500

Monoclonal

A synthesized peptide: human CD82

Human

CD82

CD82 antigen

Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.